WebTested Applications Dilution * Western Blot (WB) 1:500 - 1:2000 Immunocytochemistry (ICC) 1:200 - 1:1000 ELISA (ELISA) 1:500 - 1:2000 * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. For Research Use Only. WebThe GFP Antibody from Novus Biologicals is a chicken polyclonal antibody to GFP. This antibody reacts with jellyfish. The GFP Antibody has been validated for the following …
Anti-GFP antibody (ab92456) Abcam
WebFeb 12, 2010 · The monoclonal Anti-GFP-HRP antibody facilitates the fast and convenient detection of GFP and GFP derivatives or GFP-tagged fusion proteins in Western blot or ELISA applications. Conjugation to horseradish peroxidase (HRP) simplifies Western blot or ELISA analysis as incubation with secondary antibodies becomes dispensable. After … WebWestern Blot Protocols. Western blot (WB) is a technique that separates a mixture of proteins via size exclusion electrophoresis. The target protein is identified using an antibody specific for the target and is visualized as a band via … residences by accor
Goat anti-Chicken IgY (H+L), HRP (A16054) - Thermo Fisher Scientific
WebWestern Blotting Protocol. For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight.. NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.. A. Solutions and Reagents WebHuman 3D nucleus pulposus microtissue model to evaluate the potential of pre-conditioned nasal chondrocytes for the repair of degenerated intervertebral disc. View PDF. In Frontiers in Bioengineering and Biotechnology on 4 March 2024 by Kasamkattil, J., Gryadunova, A., et … WebBlots were blocked with 2% skim milk ON at 4°C with agitation. After rinsing with TBS, blots were incubated in the primary antibody at a dilution of 1:10 000 (anti-GFP) for 3h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed once for 15 min. and 3 times for 5 min in TBS-T at 4°C with ... protective briefs