WebAug 27, 2012 · Here we show that DEC-205, a multilectin receptor expressed by a variety of cells, is a receptor for CpG ODN. When CpG ODN are used as an adjuvant, mice deficient in DEC-205 have impaired dendritic cell (DC) and B-cell maturation, are unable to make some cytokines such as IL-12, and display suboptimal cytotoxic T-cell responses. WebCpG oligodeoxynucleotides (or CpG ODN) are short single-stranded synthetic DNA molecules containing unmethylated CpG motifs derived from bacterial DNA. CpG ODNs can directly stimulate B-lymphocytes, dendritic and NK cells, resulting in an increase in innate immunity and antibody-dependant cell cytotoxicity (ADCC).
CpG ODN2216 increases monocyte expression of CD80, CD86 …
WebMar 5, 2004 · ( E) PMBCs, CD123-depleted PMBCs, and CD123-enriched PBMCs were stimulated as in (C) and with 1 μM CpG-ODN 2216 and were tested for IFN-α secretion. One representative experiment of at least two independent experiments with two different donors is shown ( n = 2, ± SD). Open in viewer WebODN 2216 is a class A CpG ODN with a preference for human TLR9. Class A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a … garrin bucco
CpG-Induced IFN-α production of plasmacytoid dendritic …
WebSo in my hand the best way was to pre stimulate cells with PMA (10ng/ml medium) over night (approx 16 h), wash them once with medium and stimulate with CpG2216 for 5h! 2006 was not working in my... WebJan 11, 2024 · The positive control pDCs were incubated with CpG ODN 2216. As shown in Fig. 6, the administration of a blocking antibody to the IL-10 receptor is not able to neutralize the suppressive effect of the complete HNSCC supernatant on the CpG induced IFN-α secretion completely. However pDCs incubated under the influence of IL-10 can be fully ... WebMar 11, 2024 · FSC/SSChighCD4highNKp46+ cells produced high levels of IFN-α after CpG ODN 2216 stimulation, a hallmark of pDC function. Following receptor triggering with plate-bound monoclonal antibodies against NKp46, phosphorylation of signaling molecules downstream of NKp46 was analyzed in pDCs and NK cells. Comparable to NK cells, … black screen to clean