WebAug 17, 2015 · Hopefully most of you are already aware of this, but I wanted to share a few gating examples illustrating some points of concern with traditional doublet discrimination based on Ir191/193+ DNA content and event length; namely that S-phase cells fall in the same region as true doublets, and also that different cell types have different DNA … WebFor the Event Length and Gaussian parameters, CyTOFClean uses the density function to "see" the spread of the data. Where the bulk of the events are located, it will apply a …
CyTOF - Wikipedia
WebJan 1, 2024 · Mass cytometry (cytometry by time-of-flight, CyTOF, MC) is a single cell proteomics technology that allows the measure of up to 50 markers per cell. ... The event length of each cell depends on the amount of metal that the cell is loaded with, thus the non-nucleated cells with only one probe have lower event length than nucleated cells … WebThe 'Event_length' channel in CyTOF2 data is no longer being normalized. Previously the Normalizer was excluding the 'Cell_length' channel in CyTOF1 data but not the equivalent channel with the updated 'Event_length' label in the CyTOF2. For more information, see the wiki and/or the article in Cytometry. Assets 4 multi probe thermometer
Practical considerations for successful data acquisition
WebMay 5, 2016 · This fusion of the two technologies, termed mass cytometry, provides measurement of over 40 simultaneous cellular parameters at single-cell resolution, … WebJul 19, 2016 · Limitations of the CyTOF include: Slower acquisition compared to traditional flow cytometry – maximum of about 1,000 events per second Sample prep must be … WebFeb 1, 2024 · CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets. Malgorzata Nowicka 1,2, Carsten Krieg 3, Helena L. Crowell 1,2, … multiprint stone mountain ga