Hieff clone enzyme
WebHieff Clone™ Zero TOPO-TA Cloning Kit is developed by topo cloning technology, which is to achieve rapid cloning of inserts through the coupled isomerase (Topoisomerase) on the TOPO vector. This topo ta cloning kit provided by Yeasen contains TOPO-TA cloning vector and is suitable for taq-amplified PCR product WebCloneAmp™ HiFi PCR Premix Protocol-At-A-Glance (092612) www.clontech.com Clontech Laboratories, Inc. A Takara Bio Company Page 1 of 2 CloneAmp HiFi PCR Premix (Cat. …
Hieff clone enzyme
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WebEnzymes; Cells and Microorganisms; Blood and Tissue Products; Nucleic Acid Purification; Liquid Handling Workstations; Microscopes and Cell Imagers; … Web19 de jan. de 2024 · Then, AQP1 gene fragment (GenBank, NM_198098.3) was cloned into pGMLV-hTERT-MCS-EGFP using a Hieff Clone™ One Step Pcr Cloning Kit to generate pGMLV-hTERT-AQP1-EGFP plasmid. pGMLV-CMV-AQP1 ... All of the constructed plasmids were identified by restriction enzyme digestion analysis and DNA sequencing. …
WebThe following protocol walks you through the steps for designing PCR primers to seamlessly clone one or more fragments into any destination vector with In-Fusion Cloning. Use our … Web10912-A 2× Hieff Clone® MultiS Enzyme Premix 50 μL 100 μL 10912-B 500 bp Control Insert (25 ng/μL) 5 μL 5 μL 10912-C pUC 19 Control Vector, linearized (50 ng/μL) 5 μL 5 …
Web655.00. 产品描述. Hieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反 … WebGolden Gate Assembly is another method of seamless cloning that exploits the ability of Type IIS restriction enzymes (such as BsaI-HF ® v2) to cleave DNA outside of the recognition sequence. The inserts and …
Web24 de abr. de 2024 · It can influence cancer cells through inhibition of phase 1 enzymes and nuclear factor erythroid 2-related factor 2 ... (Hieff Clone Plus Multi One Step Cloning Kit, Yeasen, shanghai, China). The recombinant plasmid was verified by enzyme digestion and sequenced at the Beijing Genomics Institute.
WebBest practices. With focuses on the research and development and manufacturing of tool enzyme raw materials, antigen and antibodies, Yeasen supplies 40+ types of enzymes, … fhsc2fWeb2× Plus PCR Master Mix (With Dye) is a ready-to-use 2x premixed solution containing Hieff Canace™ Plus high-fidelity DNA polymerase, dNTPs, and an optimized buffer. This PCR … department of transportation style guideWeb23 de mar. de 2024 · From ~50,000 clones, 12 mutant strains that exhibited improved tyrosine hydroxylation capability compared with that of the strain expressing the wild-type … department of transportation tire regulationsWebOnce the Entry clone is ready, the gene of interest is easily shuttled to a secondary plasmid, the Destination vector. This reaction is mediated by a robust enzyme mixture called LR Clonase™, which contains the necessary protein activity to excise the gene of interest from the Entry clone and integrate it into the Destination vector, which then becomes your … fhs building committee ctWebThis Hieff Clone™ universal one step cloning kit is available for 1-6 fragment cloning. Products in stock in the United States can be delivered within 2 days. +1 2404726069 … department of transportation waterloo iowaWebPrimeSTAR enzyme promo; Try BcaBEST DNA Polymerase ver.2.0; RNA purification sale; Capturem IP and Co-IP sale; Baculovirus titration kits early access program; NGS bundle and save; Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit; TALON his-tag purification resin special offer; GoStix Plus special offers; PCR samples department of transportation specificationsfhsc crewe